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[Real time kinetic analysis of the 3′–>5′ exonucleolytic activity of Mg2+-dependent exonuclease of the cell free extracts of Staphylococcus aureus].
The Mg2+-dependent exonuclease of cell-free extract of S. aureus V583 was studied using real time kinetic method. It was shown that the enzyme had double-linear kinetics. The activity depended on the substrate concentration and the temperature of the reaction. The maximum activity was found at 37 degrees C (25 degrees C lower that the maximum activity at 37 degrees C). The enzyme exhibited strong processivity, only one catalytic segment was active.A woman in the United Kingdom had breast implants removed after she developed a chronic infection. The woman complained that the implants caused her right breast to be larger and sag to a lesser degree than the left breast.
Dr. Elham Safer and her colleagues at the Hospital for Special Surgery in New York City, where the woman had her implants removed, investigated this claim. The doctors observed that, over time, the tissues around the implants had lost their normal volume. The woman’s implants were surrounded by a hard lymphoid tissue that looked like a bursa. The doctors sent samples of the lymphoid tissue to the New York State Department of Health. The Department of Health confirmed the presence of Mycoplasma fermentans, a bacterium that causes breast-feeding women to develop milk allergies.
The woman had milk allergies from an early age. She was born in 1980